Lamp and Condenser project a parallel beam of light onto the sample for illumination ; Sample stage with X-Y movement sample is placed on the stage and different part of the sample can The objective forms a magnified, upside-down image, called “intermediate image”. 4x, 10x, 40x, and 100x are the most common magnifying powers used for the objectives. An optical microscope (also known as a light microscope) refers to the variant of microscopes that involve the use of visible light rays and a lens system for magnified image formation of tiny objects. The eyepiece is positioned on the optical axis in such a way that its front focal point is on the intermediate image. In this diagram, fb represents the back focal length of the objective and z’ is the optical tube length of the microscope. Olympus BX51Research Microscope Cutaway Diagram camera Beam splitter Reflected light Transmitted light 15 Functions of the Major Parts of a Optical Microscope. Its optical combination may bring-out a magnification of 50* to 1500* (* denotes linear magnification). Optical microscopes were first designed in the 17th century making them one of the oldest microscope designs. There are two basic types of optical microscopes: Simple microscopes; Compound microscopes. (a)Working of phase contrast microscope, (b) Line diagram of working od a fluorescent microscope & (c) Fluorescent treponent antibody absorption (FTA-ABS) for diagnosis of syphilis [6]. The travel path of light rays is also shown in this diagram which is self-explanatory. The optical train and numerous other components of a modern microscope are presented in a cut-away diagram in Figure 2. The sequence of components in the microscope optical train include the illuminator, condenser, specimen, objective, ocular, and camera or eye of the observer. Base: Bottom base of the microscope that houses the illumination & supports the compound microscope. Most optical microscopes are compound microscopes, which means they contain at least two lenses. Optical diagram of a microscope in the basic configuration. Optical microscopes, also called light microscopes, are the most commonly used type of microscope. The aerial intermediate image at O’ is further magnified by the microscope eyepiece (L ey) and produces an erect image of the object at O” on the retina, which appears inverted to the microscopist. The eyepiece thus functions as a magnifying behind the objective. Video: Parts of a compound Microscope with Diagram Explained 1. The condenser (containing the aperture diaphragm) and the luminous-field diaphragm normally contained in the stand base are the critical elements in achieving Köhler illumination. Picture shown below depicts a schematic arrangement of an objective and eye-piece to explain the formation of magnified image in a metallurgical microscope. Objective lenses : There are usually 3-5 optical lens objectives on a compound microscope each with different magnification levels. This section reviews the imaging and/or illuminating capability of these optical components and how they work together to … A popular method of representing a train of propagating light waves involves the application of geometrical optics to determine the size and location of images formed by a lens or multi-lens system. Simple Microscope Definition Microscope Optical Components Interactive Tutorials Geometrical Construction of Ray Diagrams. Lenses are curved pieces of glass or plastic that bend rays of light and can magnify objects, making them appear bigger than they actually are. Head (Body) The head, also referred to as the body of the microscope, is a structural component that contains the optical parts of the microscope. The figure below shows the area of the microscope considered to be the body of the microscope. 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